By K. Sowjanya Sree, Ajit Varma
This quantity describes some of the purposes of entomopathogenic soil microorganisms within the administration and keep an eye on of the devastating lepidopteran pest. An creation describes the insecticidal houses of viruses, micro organism, fungi, nematodes and their metabolites, in addition to their functions within the context of crop development. next chapters concentrate on subject matters equivalent to insecticidal proteins; the function of nucleopolyhedroviruses; Bt pollution and their receptors; regulate of lepidopterans utilizing entomopathogenic fungi; administration of cotton defoliators; and sustainable use of entomopathogenic nematodes and their bacterial symbionts. an summary of tradition collections of entomopathogenic microorganisms rounds out the volume.
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Extra resources for Biocontrol of Lepidopteran Pests: Use of Soil Microbes and their Metabolites
2001) developed a rapid method based on PCR–RFLP analysis for identification and differentiation of HaSNPV and AcMNPV groups by using a set of redundant primers to highly conserved region of polyhedrin gene. Based on REN analysis, Rovesti et al. (2000) reported that the HaNPV isolate was shown to be a mixture of many closely related genotypes but individual genotypes remained unchanged on passage in either H. armigera or M. brassicae. Doyle et al. (1990) noted that when MbMNPVD was passed in Orthosia cruda, there were minor changes in the restriction enzyme profile, which was attributed to the selection of a different variant.
2000). Differences between viruses are usually reflections of intrinsic differences in their viral genomes. At one extreme, viruses may be readily distinguished by the nature of the nucleic acid (RNA or 2 Role of Nucleopolyhedroviruses (NPVs) in the Management of Lepidopteran. . 29 DNA) and its strandedness (single or double stranded), while more closely related viruses may differ only by small regions of distinct base sequences which can be defined only by more sophisticated techniques such as restriction endonuclease (REN) analysis or molecular probes, or sequencing of conserved and unique gene sequences which offers a relatively simple method for the identification and differentiation of baculoviruses (Smith and Summers 1978).
Ignoffo (1966b) estimated that at least 6 Â 109 virus polyhedra were produced per larva in late instars of H. 5 Â 109 polyhedra (Ignoffo 1973). Teakle et al. 18 Â 107 POBs/insect was from younger larvae of H. 6 Â 109 POBs/insect from grown-up larvae, whereas Shieh (1978) recovered 5 Â 109 POBs/larva, indicating that the yield of POBs was directly related to the age of the infected larvae. The host insect, insect diet, insect age and virus dosage, incubation, environment, and preservation of virus infectivity were some of the major factors that optimize the production of HzNPV (Carter 1984).