Download Basic Malaria Microscopy, Second Edition: Part I. Learner's by UNAIDS PDF

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Some workers wipe the oil off films with absorbent tissue, but this method is rough and is not recommended. Another method is to roll examined slides in white tissue paper (toilet paper will do), with one layer of tissue paper between each slide. After a few days, when the paper has absorbed the oil, the slides can be removed from the paper. Coloured tissue should not be used as it is often acidic and will de-stain blood films. Care of the microscope Provided normal care and common sense are exercised, your microscope will remain in good condition for many years.

3. pigment in the ‘cell’? Yes: Go to 7. No: Go to 4. 4. one chromatin dot attached to a ring of blue cytoplasm containing a vacuole? Yes: This is a trophozoite. No: Go to 5. 5. one chromatin dot attached to small, solid blue cytoplasm, with no apparent vacuole? Yes: This is a trophozoite. No: Go to 6. 6. one chromatin dot and irregular or fragmented blue cytoplasm? Yes: This is a trophozoite. No: Go to 8. 7. a chromatin dot in the parasite with pigment? Yes: Go to 8. No: Go to 9. 55 Basic MALARIA MICROSCOPY Do you see: Thin film Thick film 8.

31 Basic MALARIA MICROSCOPY You will need: O buffered water in a conical flask; O the two bottles of correcting fluids; O a pH meter or a pH colour indicator; O two pH colour indicator glass cells; O one bottle of bromo-thymol-blue indicator; and O one measuring pipette, capacity 1 ml. The method: 1. Pour some of the buffered water to be tested into each of the pH colour indicator glass cells up to the 10 ml mark. 2. Place one cell in the left-hand compartment of the pH colour indicator, as the control cell.

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